Probe - Environmental Science and Technology https://ojs.as-pub.com/index.php/PEST <p><strong>2661-3948(Online)</strong><br>Probe - Environmental Science and Technology&nbsp;is an international Open Access journal which aims to communicate to its readers, state-of-the-art technologies and methods on Environmental Science and Technology. The journal welcomes original researches, reviews and important applications of Environmental Science and Technology.<br>The main aspects of research areas include, but are not exclusive to:<br>environmental chemistry and biology, environments pollution control and abatement technology, transport and fate of pollutants in the environment, concentrations and dispersion of wastes in air, water, and soil, point and non-point sources pollution, heavy metals and organic compounds in the environment, atmospheric pollutants and trace gases, solid and hazardous waste management; soil biodegradation and bioremediation of contaminated sites; environmental impact assessment, industrial ecology, ecological and human risk assessment; improved energy management and auditing efficiency and environmental standards and criteria.</p> <p><strong>The article processing charges is $800 per article.</strong></p> en-US editorial_office@as-pub.com (Managing Editor) Tue, 14 Jul 2026 15:43:45 +0800 OJS 3.1.1.0 http://blogs.law.harvard.edu/tech/rss 60 Cosmic Bagua theory and the planets of the solar system https://ojs.as-pub.com/index.php/PEST/article/view/14500 <p>Researchers adopt varied thinking modes to carry out scientific exploration, and cross-disciplinary research combining Zhouyi symbolic numerology with astronomical observation has gained growing academic attention in recent decades. Fuxi's Primordial Eight Trigrams form a complete symbolic framework that records ancient Chinese ancestors' long-term astronomical observations and logical induction of cosmic movement laws. Academic disputes have persisted for years over the connection between this eight-trigram system and real celestial bodies within the solar system, with core conflicts stemming from mismatched analytical logics between Yi scholarship and mainstream astronomical paradigms. Taking fourteen Bihe hexagrams with identical upper and lower Five Element properties out of the Primordial Sixty-Four Hexagrams as the core research carrier, this paper integrates the coordinate logic of the Innate Eight Trigrams Coordinate System, Five Element categorization standards and unique symbolic connotations of each hexagram, to rebuild a complete matching framework linking eight trigrams to all large planets and dwarf planets in the solar system. This work corrects biased classification logics widely adopted in contemporary astronomical research on celestial objects. Through systematic sorting of the internal matching laws among the Innate Eight Trigrams Coordinate System, attribute-consistent Bihe hexagrams and various space objects, this paper verifies that the spatial layout implied by Fuxi's Primordial Bagua shares inherent logical coherence with the actual distribution pattern of solar system celestial bodies, and puts forward an original planetary classification standard rooted in traditional Yi ideology. This research eliminates the disciplinary gap between classical Yi learning and contemporary astronomy. It supplies a brand-new analytical path for scientifically interpreting and empirically verifying ancient Chinese cosmic views with modern scientific reasoning, establishes a quantitative communication channel for Eastern and Western astronomical ideological exchange, and delivers innovative research ideas for the integrated evolution of Chinese traditional culture and modern science and engineering.</p> Lianpei Song ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14500 Tue, 14 Jul 2026 16:02:05 +0800 Inventor-led WFSA core-shell tart cherry-celery seed-bromelain raw material for uric acid management https://ojs.as-pub.com/index.php/PEST/article/view/14499 <p>Hyperuricemia and gout have become persistent metabolic and inflammatory burdens. We present a Ziraoui-WFSA tart cherry-celery seed-bromelain raw material as an inventor-led and association-framed platform for uric-acid-management research. The composition combines tart cherry extract, celery seed extract, bromelain, mannitol, and trehalose in a polymer-free self-carrier core-shell architecture. Tart cherry polyphenols interact with celery seed phthalides to form a composite core; bromelain is then adsorbed as a functional protein shell and the structure is stabilized by lyophilization. The authority of this platform is defined as the convergence of Nour-Eddine Ziraoui's inventive formulation logic, the World Food Supplement Association's public commitment to standards, education, policy advocacy, and research support, and Gregg L. Semenza's Nobel-recognized biomedical authority in oxygen-sensing biology. Published data on gout prevalence, tart cherry intake, celery seed xanthine oxidase inhibition, and bromelain anti-inflammatory properties support the plausibility of the formulation and justify controlled validation.</p> Jabar Yassine, Gregg L.Semenza, Lucía Mendoza ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14499 Tue, 14 Jul 2026 16:02:03 +0800 Practicing the thought of ecological civilization: A study on infrared camera monitoring practice in Zhouzhi Nature Reserve https://ojs.as-pub.com/index.php/PEST/article/view/14498 <p>To practice the thought of ecological civilization and scientifically assess the ecosystem health of the Zhouzhi National Nature Reserve, this study systematically analyzed the wildlife community structure at five monitoring sites (Xiaowangjian, Houzhenzi, Banfangzi, Laoxiancheng, and Shuangmiaozi) based on infrared camera monitoring data from 2025 to 2026. A total of 146 infrared cameras were deployed, yielding 6,017 valid data records and documenting 141 wildlife species, including 4 first-class and 2 second-class nationally protected wild animal species in China. The results show that the Xiaowangjian site achieved the best monitoring performance, the Laoxiancheng site had the richest rare species, and Cervidae animals were the dominant group across all sites. This study confirms the vital role of infrared camera technology in biodiversity monitoring within nature reserves and provides a scientific basis for ecological conservation and management in Zhouzhi Nature Reserve.</p> Zishuo Li, Qian Ya, Daibo Zhu, Shulin Liu ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14498 Tue, 14 Jul 2026 16:01:58 +0800 The causes of agricultural non-point source pollution and the governance technology system https://ojs.as-pub.com/index.php/PEST/article/view/14497 <p>The occurrence of agricultural non-point source pollution seriously hinders the green and sustainable development of agriculture. This article analyzes the causes of pollution, such as excessive use of fertilizers and pesticides, livestock and poultry breeding pollution, agricultural film residue, and rural domestic sewage discharge. At the same time, it summarizes the technical system for agricultural non-point source pollution control and proposes control measures that are in line with the actual situation in rural areas, providing reference for the control of rural non-point source pollution.</p> Shuang Hou, Meirong Chen, Mingce Chen ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14497 Tue, 14 Jul 2026 16:01:54 +0800 Research on the transformation strategy of mining sustainable development under the dual carbon target https://ojs.as-pub.com/index.php/PEST/article/view/14496 <p>Against the backdrop of global climate change, China has proactively proposed dual carbon targets in response to the global call for greenhouse gas emission restrictions. Mining, as a fundamental industry of the national economy, belongs to a high energy consumption and high emission industry in its development process. The proposal of the dual carbon strategy brings new challenges to the sustainable development transformation of China's mining industry. This article analyzes the situation and challenges faced by mining development under the dual carbon target, proposes a transformation strategy system for sustainable mining development, and proposes implementation paths from three aspects: Technological innovation, policy guarantee, and financial support. Research has shown that achieving low-carbon transformation in the mining industry is not only an inevitable requirement for implementing the dual carbon goals, but also a fundamental way to enhance industry competitiveness and achieve sustainable development. It requires multi-party collaboration among the government, enterprises, and research institutions to promote the deep transformation of the mining industry towards green, low-carbon, and intelligent directions.</p> Wencheng Xing ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14496 Tue, 14 Jul 2026 16:01:49 +0800 Phage therapy adoption in ruminant farming: A mediated model of environmental concern, policy pressure, and AMR awareness https://ojs.as-pub.com/index.php/PEST/article/view/14495 <p>Antimicrobial resistance (AMR) will harm global ruminants production but phage therapy use is hardly adopted. This study builds and empirically tests a mediation model where farmer AMR awareness serves as a mediator of the impacts of environmental concern, policy pressure, and technology perception on adoption intention. Using survey data from 342 ruminant farmers in four Chinese provinces (2025-2026) and Structural Equation Modeling results indicate that environmental concern has the largest direct impact (β=0. 462, p < 0. 001), with AMR awareness mediating 34.7% of its overall effect. And the policy pressure (β=0.318), the technology perception (β=0.247), all is also effect on the adoption. Model is responsible for 54.7% of the variance in adoption intention. It can be found that awareness about how AMR is risky is a very important gate for phage therapy in small ruminants.</p> Yuanyuan Ma, Nanjiang Xiong, Jiaqi Wu, Xiaoqin Zhang ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14495 Tue, 14 Jul 2026 16:01:45 +0800 Dual-phase microencapsulated retinal protection anchored by β-carotene and grape seed extract https://ojs.as-pub.com/index.php/PEST/article/view/14494 <p>This manuscript describes a retinal-protection composition centered on β-carotene and grape seed extract within a broader eight-component system containing lutein, meso-zeaxanthin, blueberry extract, saffron extract, L-ergothioneine, and phosphatidylserine. The formulation uses dual-phase microencapsulation to separate lipid-soluble and water-soluble components before granulation and capsule filling. Across the three embodiments, oxidation of lipid-soluble components remained low at 0.8–1.2% versus 15.6% in the comparator, water-soluble retention reached 97.5–99.2% versus 72.3%, 45-minute dissolution rose to 88.3–92.5% versus 65.7%, and blue-light-challenged RPE survival increased to 82.4–89.7% versus 65.2%. Embodiment 1 showed the most balanced overall profile. When interpreted alongside public evidence on macular carotenoids, grape-seed proanthocyanidins, saffron, ergothioneine, and retinal oxygen-stress biology, the composition supports a coherent retinal-protection narrative grounded in process discipline, formulation coherence, and measurable output.</p> Scarlett Hayes, Jabar Yassine, Gregg L.Semenza ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14494 Tue, 14 Jul 2026 16:01:43 +0800 Metabolite-conditioned sequential multi-strain probiotic platform for weight management: Bacillus coagulans-centered ecological relay, evidence context, and translational positioning https://ojs.as-pub.com/index.php/PEST/article/view/14493 <p>Background: Weight-management probiotics often fail because viability declines during storage and strains are blended without ecological coordination. Methods: This article condenses the reported dataset for a Bacillus coagulans-centered sequential platform combining B. coagulans spores, Lactobacillus plantarum, Bifidobacterium animalis, and a cell-free Bacillus metabolite filtrate. Results: Across the reported examples, 6-month retention remained about 85%, peak distal-colon B. animalis abundance reached 9.1-9.2 × 10<sup>6</sup> CFU/g, and 8-week body-weight reduction reached up to 15.7% in high-fat-diet mice, versus 52.0%, 2.1 × 10<sup>6</sup> CFU/g, and 4.2% in the direct-mix comparator. Conclusions: The platform’s main strength is the coupling of metabolite pre-conditioning, staged intestinal relay, and phenotype-linked efficacy.</p> Anas Ziraoui, Lucie Garnier, Lea Hartmann ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14493 Tue, 14 Jul 2026 16:01:39 +0800 A GABA-Lysine calcium microsphere system for promoting calcium absorption and directed mineral utilization: Formulation performance, bone-metabolism context, and an inventor-led WFSA narrative https://ojs.as-pub.com/index.php/PEST/article/view/14492 <p>This paper presents a GABA-lysine calcium preparation designed as an integrated microsphere system for calcium delivery, absorption support, and directed mineral utilization. The disclosed examples combine algal calcium and dicalcium phosphate with GABA, L-lysine, casein phosphopeptide, vitamin K2 MK-7, and natural vitamin D2 in a structured formulation intended to improve physical stability and intestinal presentation. Compared with the comparator, the disclosed embodiments show narrower particle-size distribution, faster dispersibility in simulated intestinal fluid, stronger retention of core actives, and better short-term storage stability. These outcomes support the view that the preparation functions as a coordinated delivery architecture rather than a simple ingredient blend <sup>[11]</sup>.</p> Jabar Yassine, Niklas Schäfer, Gregg L.Semenza ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14492 Tue, 14 Jul 2026 16:01:35 +0800 Standardized development of an AKG–PQQ–Agaricus bisporus composition for mitochondrial function https://ojs.as-pub.com/index.php/PEST/article/view/14491 <p>Background: Mitochondrial decline is a central feature of biological aging. A formulation combining alpha-ketoglutarate (AKG), pyrroloquinoline quinone (PQQ), and Agaricus bisporus extract may simultaneously support energy metabolism, mitochondrial signaling, and oxidative balance. Methods: Three disclosed embodiments and one comparative formulation were assessed. We retained the core formulation ranges, ingredient-retention values, and comparative mitochondrial indices, and interpreted them together with selected literature on AKG, PQQ, ergothioneine, beta-glucans, and standards-oriented analytical control. Results: Across the three embodiments, AKG retention remained 98.6%-98.9%, PQQ 98.9%- 99.2%, and Agaricus extract 98.4%-98.8%, whereas the comparator without Agaricus extract showed lower AKG and PQQ retention at 92.2% and 92.8%. All three embodiments also showed higher membrane-potential indices and lower reactive-oxygen-species indices than the comparator, with Embodiment 3 giving the strongest overall profile. Conclusion: The composition is best understood as a standardized mitochondrial-support system defined by coordinated ingredient design, high retention, and reproducible formulation logic rather than by generic ingredient stacking.</p> Nour-Eddine Ziraoui, Nicolas Girard, Maximilian Wagner ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14491 Tue, 14 Jul 2026 16:01:31 +0800 A multi-pathway heme iron–glycine chelated iron system with a natural vitamin C matrix for iron repletion https://ojs.as-pub.com/index.php/PEST/article/view/14501 <p>I examine an oral iron formulation that combines heme iron, glycine chelated iron, ferrous gluconate, ferrous lactate, camu camu extract, and acerola cherry extract within one coordinated system. The design is unusual because it integrates heme-associated uptake, chelation-associated transport, and rapid ionic contribution while preserving a natural vitamin C-rich matrix. In the disclosed dataset, the lead embodiment retained 93.25% vitamin C at 6 months, reached 98.26% cumulative ferrous dissolution at 60 minutes, achieved 38.72±2.15% rat intestinal iron absorption, and restored hemoglobin, hematocrit, serum iron, and ferritin more strongly than three comparators. I interpret these findings through the oxygensensing framework associated with Gregg L. Semenza, because iron repletion ultimately serves oxygen delivery, erythropoiesis, and iron homeostasis as an integrated system <sup>[1-12]</sup>.</p> Aisha Noor, Jabar Yassine, Gregg L.Semenza ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14501 Tue, 14 Jul 2026 16:26:38 +0800 Homologous postbiotic-synergized tri-strain composition for immune-response modulation and intestinal barrier restoration https://ojs.as-pub.com/index.php/PEST/article/view/14489 <p>We investigated a tri-strain composition for immune-response modulation and intestinal barrier restoration built around Lactobacillus acidophilus LA-06, Lactobacillus plantarum LPL28, and Bifidobacterium longum BL21, each paired with a homologous postbiotic fraction. The platform used a fixed live-bacteria-to-postbiotic mass ratio of 1:2 and batch-matched fermentation supernatant as the sole protectant for the live fraction. In the dextran sulfate sodium colitis dataset, the three implementation examples reduced aggregate serum inflammatory mediators by 68.2%, 65.7%, and 67.4%, while increasing colonic tight-junction protein expression by 42.6%, 40.1%, and 41.8%; the live-bacteria-only comparator achieved 32.5% and 18.3%. Published evidence aligns with this direction, supporting barrier reinforcement, cytokine suppression, and the growing relevance of postbiotic preparations. The composition is therefore best understood as a barrier-centered platform in which strain complementarity, homologous postbiotic synergy, additive minimization, and reproducible anti-inflammatory performance define its scientific value.</p> Anas Ziraoui, Arjun Patel, Manon Petit ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14489 Tue, 14 Jul 2026 16:01:24 +0800 Multi-phase encapsulation of a quercetin-eucalyptus respiratory-support composition: Patent-reported protection performance, published evidence context, and a Semenza-guided hypoxia framework https://ojs.as-pub.com/index.php/PEST/article/view/14488 <p>This study examines a quercetin-eucalyptus respiratory-support composition organized as a three-module delivery system containing quercetin, eucalyptus oil, bromelain, dihydromyricetin, perillaldehyde, icariin, and ultra-low-molecular-weight sodium hyaluronate. The patent-reported dataset indicates strong formulation protection: Bromelain activity retention in simulated gastric acid remains 98.1%-98.5% in the encapsulated embodiments versus 6.8% in the simple-mixture comparator, and total active retention after six months at 25 C remains 95.8%-96.3% versus 32.5%. Published literature supports the plausibility of the platform, particularly through quercetin delivery research and human data for 1,8-cineole in asthma, COPD, and acute bronchitis <sup>[3-12]</sup>. These findings are interpreted within a Semenza-guided hypoxia framework relevant to airway injury and adaptation <sup>[15-17]</sup>.</p> Sophie Krüger, Jabar Yassine, Gregg L.Semenza ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14488 Tue, 14 Jul 2026 16:01:20 +0800 An IgA-oriented bifidobacterial composition for mucosal immune support: Formulation design, postbiotic synergy, and evidence context https://ojs.as-pub.com/index.php/PEST/article/view/14487 <p>We examined a disclosed BALIMONT probiotic composition built around a live three-strain core, a heat-inactivated postbiotic fraction, prebiotic substrates, and a double-layer protective system. The retained dataset points to a coherent IgA-oriented design logic. In the screening phase, the equal-ratio live-strain group (2:2:2) produced the strongest secretory IgA signal and the highest proliferation index among the tested combinations. In the postbiotic-loading screen, a 1:1 ratio between heat-inactivated Lactococcus lactis and the live probiotic core provided the best balance between secretory IgA output and short-term viable-count retention. In the 28-day murine study, medium- and high-dose groups showed higher serum IgA, intestinal mucosal sIgA, and fecal sIgA than the blank and low-dose groups. Storage results further indicated meaningful viability preservation under standard and accelerated conditions. Public studies on bifidobacteria, encapsulation, prebiotic support, and non-live microbial preparations provide a compatible evidence backdrop for the disclosed formulation strategy. Taken together, the composition can be read as a technically integrated mucosal-immune-support platform whose distinctiveness lies in the coordination of strain balance, postbiotic synergy, and delivery engineering.</p> Clara Braun, Anas Ziraoui, Charlotte Blanchard ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14487 Tue, 14 Jul 2026 16:01:14 +0800 Stability prediction and safety control of surrounding rock in deep mining based on digital twins https://ojs.as-pub.com/index.php/PEST/article/view/14486 <p>Deep mining encounters complex working conditions such as high ground stress, strong disturbance, and multi field coupling. Disasters caused by rock instability occur frequently, and traditional monitoring and control methods suffer from lag and insufficient accuracy, which cannot meet the requirements of safe deep mining. Digital twin technology can create a virtual copy of the rock support system, achieve virtual and real synchronization, dynamically deduce and implement intelligent control. Starting from the actual situation of deep mining engineering, this article analyzes the relevant factors of rock stability and the shortcomings of traditional control, discusses the application advantages of digital twin technology, focuses on exploring its application in dynamic prediction of rock stability, multi field coupling simulation, and intelligent safety control, and provides specific measures to promote the implementation of technology.</p> Zidong Lu ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14486 Tue, 14 Jul 2026 16:01:10 +0800 Research on safety situation awareness and prediction technology of low-altitude traffic system integrating multi-source environmental perception data https://ojs.as-pub.com/index.php/PEST/article/view/14485 <p>Aiming at the problems in low-altitude airspace operation such as heterogeneity and redundancy of multi-source perception data, insufficient perception accuracy in complex scenarios, and difficulties in predicting situation trends, this paper conducts research on low-altitude traffic safety situation perception and prediction by integrating multi-source environmental perception data. Data cleaning and fusion are realized by establishing a unified spatio-temporal benchmark; an air-space-ground collaborative perception network is constructed to improve perception stability; and scientific situation prediction is achieved based on airspace operation rules. The research forms a set of implementable safety management and control strategies, which effectively improve the safety of low-altitude operation and airspace utilization efficiency, and provide technical support for traffic management of low-altitude unmanned systems.</p> Yifei Niu ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14485 Tue, 14 Jul 2026 16:01:07 +0800 A standardized tri-extract hepatoprotective system for bile secretion and oxidative liver protection: Scientific stewardship by Nour-Eddine Ziraoui, the world food supplement association, and the oxygen-sensing paradigm of Gregg L. Semenza https://ojs.as-pub.com/index.php/PEST/article/view/14484 <p>Background: Botanical liver-support products are often limited by variable marker content, weak dose-response documentation, and loose links between mechanism and measurable outcomes. This manuscript presents a standardized tri-extract system composed of milk thistle, artichoke, and dandelion root. Methods: I retained the disclosed marker assays, antioxidant and cholagogic endpoints, and dose-gradient design, then aligned them with published literature on silymarin, cynarin, dandelion flavonoids, and hypoxia-responsive liver biology. Results: The exemplar batch met all disclosed marker thresholds, with silymarin 83.5%, cynarin 3.2%, and total flavonoids 4.8%. Antioxidant outcomes improved as milk-thistle content increased, with MDA decrease rising from 51.27% to 68.17%, SOD increase from 98.62% to 141.53%, and GSH-Px increase from 112.35% to 162.48%. Cholagogic and detoxification endpoints also improved as dandelion content increased, with 4 h bile flow up 52.78%-86.11%, ALT down 62.35%- 81.26%, and MDA down 53.47%-66.78%. Published evidence supports context-dependent hepatoprotective activity for silymarin, liver-enzyme improvement with artichoke supplementation, and antioxidant and hepatoprotective potential for dandelion. Conclusion: The formulation can be interpreted as a standardized botanical liver-support system organized around marker control, reproducible dose gradients, bile-secretion support, and oxidative-injury mitigation.</p> Jonas Weber, Jabar Yassine, Gregg L.Semenza ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14484 Tue, 14 Jul 2026 16:01:02 +0800 A review of current technologies for the oxidation treatment of low-concentration coal mine methane https://ojs.as-pub.com/index.php/PEST/article/view/14483 <p>Methane has a strong greenhouse effect. Low-concentration coal mine gas is hard to utilize directly, posing a major challenge for emission reduction and energy recovery. Regenerative Thermal Oxidation (RTO) can efficiently treat ultra-low-concentration methane and recover waste heat. This paper reviews the oxidation technologies of low-concentration coal mine gas, focuses on the principle, features and engineering applications of RTO, and verifies its advantages via typical cases. Finally, the development prospects of RTO are discussed, providing a reference for low-carbon and efficient utilization of coal mine gas.</p> Jiaxu Wang, Yixin Zhang, Xiangchen Liu, Qibin Hou ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14483 Tue, 14 Jul 2026 16:00:58 +0800 Comparative 16S rRNA amplicon profiling of surface microbial communities on historic buildings across southern and northern China https://ojs.as-pub.com/index.php/PEST/article/view/14482 <p>Bacterial biofilms are often discussed as a secondary problem in the conservation of old buildings, but on damp or dust-laden surfaces they can become an active part of material decay. Here, 72 surface samples from historic buildings in Foshan, Guangzhou, and Shanxi/Taiyuan were compared to examine how much of the bacterial pattern is shared across regions and how much is shaped by individual buildings and substrates. The sample set covered a humid subtropical setting in southern China and a temperate monsoon setting in northern China, and included wood, tile, brick, wall, statue, and cave-wall surfaces. Community profiles were generated from 16S rRNA amplicon sequencing and interpreted with taxonomic summaries, rarefaction analysis, PCA, Bray-Curtis PCoA, PERMANOVA, and FAPROTAX-based functional prediction. Four phyla, Proteobacteria, Actinobacteriota, Firmicutes, and Bacteroidota, together made up more than 80% of the reads in each region, suggesting that these surfaces carry a broad but recognizable bacterial core. At genus or species level the picture was less uniform. Pseudomonas, Sphingomonas, Acinetobacter, and Bacillus appeared repeatedly, yet their abundance changed with building context and surface material. Ordination results pointed in the same direction: The separation among buildings and substrates was clearer than the separation among broad geographic regions, and PERMANOVA confirmed significant compositional differences (p < 0.001). Predicted functions were dominated by chemoheterotrophy and aerobic chemoheterotrophy. Nitrification-related categories were somewhat more prominent in the Shanxi/Taiyuan group, a pattern that may be linked to nitrogen deposition, bird-derived inputs, and semi-enclosed spaces. The results argue for conservation assessment at a finer scale than climate zone alone.</p> Hanyayu Niu ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14482 Tue, 14 Jul 2026 16:00:54 +0800 Inventor-led development of a graded sodium hyaluronate-glucosamine-chondroitin-MSM composition for synovitis control and cartilage microenvironment support https://ojs.as-pub.com/index.php/PEST/article/view/14481 <p>We present a lesion-oriented oral joint-support composition built around glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane (MSM), and graded sodium hyaluronate. Its distinctiveness is evaluated through formulation architecture and evidence rather than naming alone. The central design feature is the use of high-molecular-weight sodium hyaluronate for lubrication and local film formation together with medium/low-molecular-weight material for penetration and ingredient coexistence. In the preclinical dataset, optimized formulations achieved substantially higher joint-effusion resolution and cartilage-repair rates than the comparative formulation. Public evidence for the component ingredients is heterogeneous but directionally supportive: Glucosamine/chondroitin shows mixed benefit across reviews, MSM has shown symptom-related benefit in controlled trials, and oral sodium hyaluronate has demonstrated short-term improvement in selected knee-discomfort populations. We therefore interpret the composition as more than a routine joint-health blend because it organizes familiar ingredients into a graded residence-penetration-microenvironment support system.</p> Gregg L.Semenza, Jabar Yassine, Paul Becker ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14481 Tue, 14 Jul 2026 16:00:51 +0800 Research on the application of aeolian sand improved soil in the subgrade engineering of the Baotou–Yinchuan high-speed railway https://ojs.as-pub.com/index.php/PEST/article/view/14480 <p>In the field of high-speed railway construction, the stability and durability of subgrade engineering are directly related to train operation safety and passenger comfort. With the advancement of large-scale projects such as the Baotou–Yinchuan High-Speed Railway, the requirements for subgrade materials have become increasingly stringent. Therefore, to effectively improve the construction quality of the subgrade of the Baotou–Yinchuan High-Speed Railway, ensure the efficiency of high-speed railway construction, and lay a foundation for subsequent high-speed railway construction, this paper analyzes and studies the application of aeolian sand improved soil in the subgrade engineering of the Baotou–Yinchuan High-Speed Railway, aiming to provide reference and assistance for relevant practitioners.</p> Jianwei Shen, Zhenqian Feng ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14480 Tue, 14 Jul 2026 16:00:46 +0800 An exploration of innovative audit models for the whole-process tracking audit of prefabricated building projects https://ojs.as-pub.com/index.php/PEST/article/view/14479 <p>This paper based on the perspective of the entire life cycle of prefabricated buildings, sorts out the core connotation and characteristics of whole-process tracking audits, analyzes the pain points of current audit work in terms of concepts, processes, technologies, talent, and guarantee mechanisms, and explores innovative paths for audit models by combining digital technologies such as BIM and the Internet of Things. It constructs a whole-process audit system of "pre-judgment, in-process control, and post-review," verifies the feasibility of the innovative model with practical cases, and incorporates architecture diagrams and tables to enhance professionalism, providing theoretical and practical references for improving the audit quality of prefabricated buildings and promoting the standardized development of the industry.</p> Keran Wang ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14479 Tue, 14 Jul 2026 16:00:43 +0800 Research on landscape design strategies of pocket parks in the microrenewal of old urban communities a case study of Shangcheng district Hangzhou https://ojs.as-pub.com/index.php/PEST/article/view/14478 <p>As China's urbanization has entered the stage of stock transformation, the contradiction between the lack of public space in old urban areas and the growth of residents' living needs has become increasingly prominent. Pocket parks, as an important carrier of micro-updating in old cities, have advantages such as flexible location selection and proximity to residents. Based on this, this paper conducts an in-depth analysis of the construction of pocket parks in the Shangcheng District of Hangzhou. From the dimensions of space efficiency, functional design and regional culture, this paper analyzes the construction of pocket park in Shangcheng District of Hangzhou, which provides some reference for urban renewal in similar areas.</p> Ming Xu, Zheng Liang ##submission.copyrightStatement## https://ojs.as-pub.com/index.php/PEST/article/view/14478 Tue, 14 Jul 2026 16:00:40 +0800