Published
2025-10-15
Section
Articles
How to Cite
丝状真菌疏水蛋白的表达调控机制研究进展
吴 斯凯
福建师范大学生命科学学院 工业微生物发酵技术国家地方联合工程研究中心
江 贤章
福建师范大学生命科学学院 工业微生物发酵技术国家地方联合工程研究中心
DOI: https://doi.org/10.59429/zxyy.v2i4.11693
Keywords: 丝状真菌；疏水蛋白；转录调控；翻译后修饰
Abstract
丝状真菌疏水蛋白是具独特两亲性的表面活性蛋白，在真菌生长发育与环境适应中作用关键。随着相关技术 发展，其表达调控机制研究取得显著进展。本文梳理了其结构、功能及工业应用潜力，重点分析关键调控因素，指 出其表达受转录、翻译及翻译后修饰等多层次调控：转录层面有转录因子和环境因素影响，翻译后修饰（尤其糖基 化）对其稳定性和功能至关重要。目前研究存在调控网络认识不足等问题，未来需加强多组学整合，为其在多领域 应用提供理论支撑。
References
[1] KHALESI M, GEBRUERS K, DERDELINCKX G. Recent Advances in Fungal Hydrophobin Towards Using in Industry[J]. The Protein Journal, 2015, 34(4): 243-255.
[2] VEREMAN J, THYSENS T, VAN IMPE J, etl al. Improved extraction and purification of the hydrophobin HFBI[J]. Biotechnology Journal, 2021, 16(11): e2100245.
[3] BELL‐PEDERSEN D, LEWIS Z A, LOROS J J, etl al. The Neurospora circadian clock regulates a transcription factor that controls rhythmic expression of the output eas(ccg‐2) gene[J]. Molecular Microbiology, 2001, 41(4): 897-909.
[4] WÖSTEN H A B, VAN WETTER M A, LUGONES L G, etl al. How a fungus escapes the water to grow into the air[J]. Current Biology, 1999, 9(2): 85-88.
[5] WHITEFORD J R, SPANU P D. The Hydrophobin HCf-1 of Cladosporium fulvum Is Required for Efficient Water-Mediated Dispersal of Conidia[J]. Fungal Genetics and Biology, 2001, 32(3): 159-168.
[6] GUZMÁN-GUZMÁN P, ALEMÁN-DUARTE M I, DELAYE L, etl al. Identification of effector-like proteins in Trichoderma spp. and role of a hydrophobin in the plantfungus interaction and mycoparasitism[J]. BMC Genetics, 2017, 18(1).
[7] BROMLEY K M, MORRIS R J, HOBLEY L, etl al. Interfacial self-assembly of a bacterial hydrophobin[J]. Proceedings of the National Academy of Sciences, 2015,
112(17): 5419-5424.
[8] ZHI M, ZHAO Y, ZENG X, etl al. Filamentous cyanobacteria and hydrophobic protein in extracellular polymeric substances facilitate algae—bacteria aggregation during partial nitrification[J]. International Journal of Biological Macromolecules, 2023, 251: 126379.
[9] QIAO J, LIU H, XUE P, etl al. Function of a hydrophobin in growth and development, nitrogen regulation, and abiotic stress resistance of Ganoderma lucidum[J]. FEMS Microbiology Letters, 2023, 370.
[10] ZHAO L, XU H, LI Y, etl al. Novel application of hydrophobin in medical science: a drug carrier for improving serum stability[J]. Scientific Reports, 2016, 6(1): 26461. DOI:10.1038/srep26461.
[11] TANIGUCHI S, SANDIFORD L, COOPER M, etl al. Hydrophobin-Encapsulated Quantum Dots[J]. ACS Applied Materials & Interfaces, 2016, 8(7): 4887-4893.
[12] AL‐TERKE H H, BEAUNE G, JUNAID M, etl al. Hydrophobin‐Coated Echogenic Microbubbles for Molecular Targeting of Tumor Cells[J]. Advanced Science, 2025, 12(22): 2401526.
[13] MAIOLO D, PIGLIACELLI C, SÁNCHEZ MORENO P, etl al. Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release[J]. ACS Nano,
2017, 11(9): 9413-9423.
[14] BOEUF S, THROM T, GUTT B, etl al. Engineering hydrophobin DewA to generate surfaces that enhance adhesion of human but not bacterial cells[J]. Acta Biomaterialia, 2012, 8(3): 1037-1047.
[15] NAKARI‐SETÄLÄ T, ARO N, ILMÉN M, etl al. Differential Expression of the Vegetative and Spore‐Bound Hydrophobins of Trichoderma Reesei Cloning and
Characterization of the Hfb2 Gene[J]. European Journal of Biochemistry, 1997, 248(2): 415-423.
[16] LI X, LIU M, DONG C. Hydrophobin Gene Cmhyd4 Negatively Regulates Fruiting Body Development in Edible Fungi Cordyceps militaris[J]. International Journal of Molecular Sciences, 2023, 24(5): 4586.
[17] HAN J, KAWAUCHI M, TERAUCHI Y, etl al. Physiological function of hydrophobin Vmh3 in lignin degradation by white-rot fungus Pleurotus ostreatus[J]. Letters in Applied Microbiology, 2023, 76(4): 048.
[18] MIKUS M, HATVANI L, NEUHOF T, etl al. Differential Regulation and Posttranslational Processing of the Class II Hydrophobin Genes from the Biocontrol
FungusHypocrea atroviridis[J]. Applied and Environmental Microbiology, 2009, 75(10): 3222-3229.
[19] WESSELS J G H. Hydrophobin Genes lnvolved in Formation of Aerial Hyphae and Fruit Bodies in Schizophyllum[J]. Plant Cell. 1991 Aug;3(8):793-799.
[20] LUCIANO-ROSARIO D, EAGAN J L, ARYAL N, etl al. The Hydrophobin Gene Family Confers a Fitness Trade-off between Spore Dispersal and Host Colonization in Penicillium expansum[J]. mBio, 2022, 13(6).
[21] RAFEEQ C M, VAISHNAV A B, MANZUR ALI P P. Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)[J]. Protein Expression and Purification, 2021, 182: 105834.
[22] ZHANG X, ZHAO L, LIU S, etl al. Identification and Functional Analysis of a Novel Hydrophobic Protein VdHP1 from Verticillium dahliae[J]. Microbiology Spectrum, 2022, 10(2).
[23] LUO X, AFFELDT K J, KELLER N P. Characterization of the Far Transcription Factor Family in Aspergillus flavus[J]. G3 Genes|Genomes|Genetics, 2016, 6(10): 3269-3281.
[24] YAP H Y Y, CHOOI Y H, FUNG S Y, etl al. Transcriptome Analysis Revealed Highly Expressed Genes Encoding Secondary Metabolite Pathways and Small CysteineRich Proteins in the Sclerotium of Lignosus rhinocerotis[J]. PLOS ONE, 2015, 10(11): e0143549.
[25] SALLADA N D, DUNN K J, BERGER B W. A Structural and Functional Role for Disulfide Bonds in a Class II Hydrophobin[J]. Biochemistry, 2018, 57(5): 645-653.
[26] HU X, ZHOU Y, LIU R, etl al. Protein disulfide isomerase 1 is required for RodA assembling-based conidial hydrophobicity of Aspergillus fumigatus[J]. Applied and Environmental Microbiology, 2024, 90(4).
[27] NAKARI‐SETÄLÄ T, ARO N, KALKKINEN N, etl al. Genetic and Biochemical Characterization of the Trichoderma Reesei Hydrophobin HFBI[J]. European Journal
of Biochemistry, 1996, 235(1-2): 248-255.
[28] SALLADA N D, HARKINS L E, BERGER B W. Effect of gene copy number and chaperone coexpression on recombinant hydrophobin HFBI biosurfactant production in Pichia pastoris[J]. Biotechnology and Bioengineering, 2019, 116(8): 2029-2040.
[29] HAN J, KAWAUCHI M, SCHIPHOF K, etl al. Features of disruption mutants of genes encoding for hydrophobin Vmh2 and Vmh3 in mycelial formation and resistance to environmental stress in Pleurotus ostreatus[J]. FEMS Microbiology Letters, 2023, 370.