Published
2026-05-12
Section
Articles
How to Cite
大鼠脑组织中全氟壬酸的LC-MS/MS分析方法的建立与验证
张 宸祎
中国药科大学药学院
罗 传梦
中国药科大学药学院
张 蓉
中国药科大学药学院
陆 宇婷
中国药科大学药学院
DOI: https://doi.org/10.59429/zxyy.v3i2.13910
Keywords: 全氟壬酸；脑组织；LC-MS/MS
Abstract
本研究通过系统优化色谱条件、质谱参数及样品前处理步骤，建立了适用于脑组织中全氟壬酸（PFNA）的LC-MS/MS 检测方法。样品前处理采用 0.2% 甲酸酸化 - 乙腈蛋白沉淀法，并以13C9-PFNA 作为同位素内标。色谱分离使用 HypersilTM BDS C18 色谱柱（100 mm×4.6 mm，2.4 μm），流动相为 2 mM 乙酸铵水溶液 - 甲醇，进行梯度洗脱，流速为 0.7 mL·min-1。对该方法的专属性、线性范围、精密度、准确度、基质效应及稳定性进行了全面验证，均符合生物样本分析要求，并成功应用于大鼠脑组织样本检测。
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