Extracellular Enzyme-Producing Bacteria from Soil and Poultry Waste: Industrial and Environmental Potential
Adiatuj Jahan Rimu
Adiatuj Jahan Rimu, Department of Public Health & Life Sciences, University of South Asia, Dhaka, Bangladesh
Israt Binta Isa
Israt Binta Isa, Department of Microbiology Laboratory, Mohila O Shishu Hospital, Uttara, Dhaka, Bangladesh
Afroj Jahan Bristi
Afroj Jahan Bristi, Department of Horticulture, Sylhet Agricultural University, Bangladesh
Md. Al-Amin Hossen
Md. Al-Amin Hossen, Department of Pathology, United Hospital Limited, Dhaka, Bangladesh
DOI: https://doi.org/10.59429/ersr.v3i1.11823
Keywords: Extracellular enzymes; amylase, cellulase; lipase; soil and poultry waste; industrial biotechnology
Abstract
Microbial enzymes play a crucial role in industrial and environmental biotechnology due to their biodegradability, specificity, and ability to function under mild conditions. This study aimed to isolate and characterize extracellular enzyme-producing bacteria from soil and poultry waste collected in Mirer Bazar, Tongi, Bangladesh. A total of 40 bacterial isolates were screened for amylase, cellulase, pectinase, lipase, and protease activities through primary plate assays (Table 1) and subsequent shake-flask fermentation experiments (Tables 2–6). Significant variation in enzyme production was observed among isolates. Strong amylase and cellulase activity were detected in PLW 5EH, PLW 5FH, GRDF 2, PEDF 10⁻³ 1, and PLW 5CH, with halo diameters reaching 40–46 mm, indicating their potential for starch and cellulose hydrolysis in industrial processes such as food processing, biomass conversion, and biofuel production. Moderate lipase activity was observed in PLW 1AH and PLW 2CH, suggesting their suitability for lipid degradation and biocatalysis applications. Pectinase and protease activities were generally low, implying that optimized culture conditions or substrate induction may be required for enhanced production. The study highlights the diversity of enzyme-producing bacteria from environmental sources and identifies promising strains for industrial and environmental biotechnological applications. Future research should focus on optimizing fermentation conditions, enzyme purification, biochemical characterization, and molecular identification of top-performing isolates.
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