Grassland Vegetation-Soil-Enzyme Responses to Gold Mineralization Anomalies: Implications for Ecological Restoration and Net-Zero Ecosystem Management
Masroor Ahmed
Department of Environmental Science and Engineering, Chengdu University of Technology, Chengdu, China
Prabal Barua
Departments of Environmental Sciences, Jahangirnagar University, Savar, Dhaka, Bangladesh
Nahida Nargis
Departments of Environmental Science and Engineering, Chang'an University, Shaanxi, China
Noor Mohammad
Department of Organizational Operation and Human Resources Management, Chang’an University, Shaanxi, China
DOI: https://doi.org/10.59429/ersr.v4i1.15064
Keywords: gold mineralization, grassland ecosystems, soil enzymes, vegetation–soil interactions, metal bioavailability, ecological restoration, soil carbon
Abstract
Gold-mineralized grasslands may contain spatially variable gold (Au) and associated elements such as arsenic (As) and mercury (Hg). Ecological effects are difficult to attribute because natural mineralization, mining disturbance and other sources of metal contamination may overlap. This narrative review examines vegetation, soil geochemistry and microbial enzyme activity in grasslands and related metal-affected systems. The available studies are heterogeneous and often do not measure Au, vegetation, soil properties and enzyme activity together. Studies that do not measure Au are treated as contextual evidence, not direct evidence of Au-specific effects. Unsupported cross-site Au concentrations, pooled regolith statistics, universal particle-size or dispersion ranges, plant accumulation values and enzyme diagnostic thresholds have been removed. Enzyme activities are complementary indicators of soil biological functioning, not validated Au-specific diagnostics. Restoration evidence from other ecosystems can inform hypotheses but cannot establish universal restoration outcomes for naturally mineralized grasslands. Changes in enzyme activity or SOC alone do not demonstrate net-zero performance; assessment requires repeated SOC-stock measurements, plant carbon inputs, CO₂, CH₄ and N₂O fluxes, and consideration of restoration-related emissions. The review therefore presents a conceptual monitoring agenda and testable research priorities rather than a validated causal model or universal thresholds.
References
1.Angelovicova, L., Lodenius, M., Tulisalo, E., & Fazekasova, D. (2014). Effect of heavy metals on soil enzyme activity at different field conditions in Middle Spis mining area (Slovakia). Bull Environ Contam Toxicol, 93(6), 670-675. https://doi.org/10.1007/s00128-014-1397-0
2.Bhaduri, D., Sihi, D., Bhowmik, A., Verma, B. C., Munda, S., & Dari, B. (2022). A review on effective soil health bio-indicators for ecosystem restoration and sustainability. Front Microbiol, 13, 938481. https://doi.org/10.3389/fmicb.2022.938481
3.Bi, Y., Guo, N., Zhang, Y., Li, X., & Song, Z. (2024). Responses of soil stoichiometry and soil enzyme activities in the different distance around opencast coal mine of the Hulun Buir Grassland of China. International Journal of Coal Science & Technology, 11(1). https://doi.org/10.1007/s40789-024-00662-4
4.Blake, F., Grant, K., MacKenzie, D., Scott, J., & Craw, D. (2019). Surficial arsenic redistribution above gold-mineralised zones in East Otago, New Zealand. New Zealand Journal of Geology and Geophysics, 62(4), 573-587. https://doi.org/10.1080/00288306.2019.1647854
5.Campillo-Cora, C., Rodríguez-Seijo, A., Pérez-Rodríguez, P., Fernández-Calviño, D., & Santás-Miguel, V. (2025). Effect of heavy metal pollution on soil microorganisms: Influence of soil physicochemical properties. A systematic review. European Journal of Soil Biology, 124, 103706. https://doi.org/10.1016/j.ejsobi.2024.103706
6.Daunoras, J., Kacergius, A., & Gudiukaite, R. (2024). Role of Soil Microbiota Enzymes in Soil Health and Activity Changes Depending on Climate Change and the Type of Soil Ecosystem. Biology (Basel), 13(2). https://doi.org/10.3390/biology13020085
7.Deng, J., Wang, Y., Yu, D., Li, X., & Yue, J. (2025). Effects of heavy metals on variation in bacterial communities in farmland soil of tailing dam collapse area. Sci Rep, 15(1), 8100. https://doi.org/10.1038/s41598-025-93244-6
8.Ebyan, O. (2024). Biogeochemical Exploration for Gold Mineralization Using Wild Plants. Journal of the Turkish Chemical Society Section A: Chemistry, 11(3), 1125-1140. https://doi.org/10.18596/jotcsa.1421730
9.FAO. (2022). The State of the World's Land and Water Resources for Food and Agriculture 2022. Food and Agriculture Organization of the United Nations.
10.Fashola, M. O., Ngole-Jeme, V. M., & Babalola, O. O. (2016). Heavy Metal Pollution from Gold Mines: Environmental Effects and Bacterial Strategies for Resistance. Int J Environ Res Public Health, 13(11). https://doi.org/10.3390/ijerph13111047
11.Gan, F., Shi, H., Tan, X., Jiang, L., Li, W., Xia, Y., Pu, J., Dai, Q., Yan, Y., & Fan, Y. (2025). Responses of Soil Enzyme Activity and Microbial Nutrient Limitations to Vegetation Types in a Degraded Karst Trough Valley. Forests, 16(2). https://doi.org/10.3390/f16020279
12.Garaiyurrebaso, O., Garbisu, C., Blanco, F., Epelde, L., & Becerril, J. M. (2017). Long-term effects of aided phytostabilisation on microbial communities of metal-contaminated mine soil. FEMS Microbiology Ecology, 93(3), fiw252. https://doi.org/10.1093/femsec/fiw252
13.Gimenez, M., Berenstecher, P., Ligrone, A., Iraola, G., & Pineiro, G. (2025). Soil microbiome analysis of Uruguayan grasslands and croplands reveals losses of microbial diversity and necromass recycling traits. Environ Microbiome, 20(1), 96. https://doi.org/10.1186/s40793-025-00696-4
14.Gong, J., Sun, F., Wu, J., Li, Y., & Yang, Y. (2025). Long-term active rather than passive restoration promotes soil organic carbon accumulation by alleviating microbial nitrogen limitation in an extremely degraded alpine grassland. Advanced Science, e10549. https://doi.org/10.1002/advs.202510549
15.Guasconi, D., Cousins, S. A. O., Manzoni, S., Roth, N., & Hugelius, G. (2025). Experimental drought and soil amendments affect grassland above- and belowground vegetation but not soil carbon stocks. Soil, 11(1), 233-246. https://doi.org/10.5194/soil-11-233-2025
16.Haghighizadeh, A., Rajabi, O., Nezarat, A., & Karimi, H. (2024). Comprehensive analysis of heavy metal soil contamination in mining environments: Impacts, monitoring techniques, and remediation strategies. Arabian Journal of Chemistry, 17(6), 105777. https://doi.org/10.1016/j.arabjc.2024.105777
17.Li, X., An, X., Jiao, K., Pan, H., & Lian, B. (2025). Current state of the heavy metal pollution, microbial diversity, and bioremediation experiments around the Qixia Mountain lead-zinc mine in Nanjing, China. RSC Adv, 15(11), 8795-8808. https://doi.org/10.1039/d4ra07920e
18.Li, Y., Zhao, L., Hilal, M. G., Guo, L., Zhang, Y., Ji, Y., Jiang, X., Hao, L., & Lin, K. (2024). Effects of grassland degradation on soil ecological stoichiometry and soil microbial community on the South of the Greater Khingan Mountains. Front Microbiol, 15, 1438787. https://doi.org/10.3389/fmicb.2024.1438787
19.Liu, H., Ren, T., Liao, J., Chen, H. Y. H., Zou, X., & Ruan, H. (2025). Nitrogen deposition alleviates phosphorus-induced imbalances in soil enzyme stoichiometry. Communications Earth & Environment. https://doi.org/10.1038/s43247-025-03115-1
20.Liu, Z., Shen, Y., Wang, G., Li, H., Ma, J., Zhou, Y., Li, W., & Ma, H. (2025). Moderate time grazing in the warm season maintained the diversity and complexity of soil microorganisms by regulating nutrient cycling and decomposition function. Functional Ecology, 39(10), 2772-2789. https://doi.org/10.1111/1365-2435.70131
21.López-Mateo, C., Marcos-Rodríguez, R., Díaz-Rodríguez, F., & Fernandez-Marcos, M. L. (2023). Forms of Toxic and Trace Metals in Grassland Soils of Galicia, Spain. Spanish Journal of Soil Science, 13. https://doi.org/10.3389/sjss.2023.11201
22.Lupwayi, N. Z., Hao, X., & Gorzelak, M. A. (2024). Divergent responses of the native grassland soil microbiome to heavy grazing between spring and fall. Microbiology (Reading), 170(11). https://doi.org/10.1099/mic.0.001517
23.Ma, R., Kong, J., Mou, H., & Liu, B. (2025). Mine drainage leads to the reshaping of the spatial patterns of soil extracellular econzymatic stoichiometry and microbial resource limitation. Sci Rep, 15(1), 42844. https://doi.org/10.1038/s41598-025-20858-1
24.McLagan, D. S., Eboigbe, E. O., & Strickman, R. J. (2025). Reviews and syntheses: Artisanal and small-scale gold mining (ASGM)-derived mercury contamination in agricultural systems. Biogeosciences, 22, 6695–6726. https://doi.org/10.5194/bg-22-6695-2025
25.Medina-Ruiz, A., Jimenez-Millan, J., Abad, I., & Jimenez-Espinosa, R. (2024). Anomalous Gold Concentrations in Hypersaline Wetland Sediments (Laguna Honda, South Spain) Caused by Nanoparticles Used in Agricultural Practices: Environmental Transformation. Toxics, 12(3). https://doi.org/10.3390/toxics12030223
26.Newsome, L., & Falagan, C. (2021). The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health. Geohealth, 5(10), e2020GH000380. https://doi.org/10.1029/2020GH000380
27.Nikolova, R., Boteva, S., Kenarova, A., & Hristova, M. (2023). Enzyme activities in soils under heavy metal pollution. Biotechnology & Biotechnological Equipment, 37(1), 49–57. https://doi.org/10.1080/13102818.2022.2164594
28.Peijnenburg, W. J. G. M. (2025). Bioavailability of heavy metals in soil: a review of tools, models, and regulatory applications. Environmental and Biogeochemical Processes, 1(1), 0-0. https://doi.org/10.48130/ebp-0025-0011
29.Qin, H., & Wang, Z. (2021). Biogeochemistry of Dominant Plants and Soils in Shewushan Gold Lateritic Deposit, China. Plants (Basel), 11(1). https://doi.org/10.3390/plants11010038
30.Shu, X., Hu, Y., Liu, W., Xia, L., Zhang, Y., Zhou, W., Liu, W., & Zhang, Y. (2023). Linking between soil properties, bacterial communities, enzyme activities, and soil organic carbon mineralization under ecological restoration in an alpine degraded grassland. Front Microbiol, 14, 1131836. https://doi.org/10.3389/fmicb.2023.1131836
31.Sun, R., Wang, Z., & Zhou, X. (2024). Soil extracellular enzyme stoichiometry reveals microbial nutrient constraints in degraded grasslands. Applied Soil Ecology, 195, 105123. https://doi.org/10.1016/j.apsoil.2023.105123
32.Száková, J., Stefanović, L., Robledo Mahón, T., Khaustov, A., Cieslarová, A., Tejnecký, V., Sysalová, J., & Tlustoš, P. (2025). Risk elements in soils affected by long-term gold and mercury (Hg) mining related to soil microbial activity. Chemistry and Ecology, 41(5), 694-709. https://doi.org/10.1080/02757540.2025.2468947
33.UNEP. (2024). Nature-Based Solutions for Net-Zero: Grassland Carbon Pathways. Nairobi: United Nations Environment Programme.
34.van Wyk, S. J., & Haagner, A. S. H. (2023). Soil Microbial Enzymatic Activity in 20-year-old rehabilitated platinum tailings substrates in South Africa. *Mine Closure 2023: Proceedings of the 16th International Conference on Mine Closure, Reno, Nevada, USA* (Paper 051, pp. 1-13). Australian Centre for Geomechanics, Perth. ISBN 978-0-6450938-7-2. https://doi.org/10.36487/ACG_repo/2315_051
35.Wan, Y., Liu, J., Zhuang, Z., Wang, Q., & Li, H. (2024). Heavy Metals in Agricultural Soils: Sources, Influencing Factors, and Remediation Strategies. Toxics, 12(1). https://doi.org/10.3390/toxics12010063
36.Wang, L., Hamel, C., Lu, P., Wang, J., Sun, D., Wang, Y., Lee, S. J., & Gan, G. Y. (2023). Using enzyme activities as an indicator of soil fertility in grassland - an academic dilemma. Front Plant Sci, 14, 1175946. https://doi.org/10.3389/fpls.2023.1175946
37.Wang, X., Xia, W., Yan, K., Yu, K., Wang, J., Yang, X., Zhou, Y., Zhang, H., Jiang, L., Han, X., Sun, O. J., Jiang, Y., & Yu, L. (2025). Differential impacts of nitrogen compounds on soil acid phosphatase activity in a meadow steppe. Ecological Processes, 14(1). https://doi.org/10.1186/s13717-025-00597-x
38.Wang, Z., Li, X., Ji, B., Struik, P. C., Jin, K., & Tang, S. (2021). Coupling Between the Responses of Plants, Soil, and Microorganisms Following Grazing Exclusion in an Overgrazed Grassland. Front Plant Sci, 12, 640789. https://doi.org/10.3389/fpls.2021.640789
39.Xie, Y., Fan, J., Zhu, W., Amombo, E., Lou, Y., Chen, L., & Fu, J. (2016). Effect of Heavy Metals Pollution on Soil Microbial Diversity and Bermudagrass Genetic Variation. Front Plant Sci, 7, 755. https://doi.org/10.3389/fpls.2016.00755
40.Yu, X., Li, T., Wu, X., Chen, Y., & Zhao, L. (2025). Exudates of dominant plants regulate rhizospheric soil total and available heavy metals and facilitate natural restoration succession in an abandoned metal mining area. Frontiers in Environmental Science, 13, 1698742. https://doi.org/10.3389/fenvs.2025.1698742
41.Yuan, X., Wang, Y., Li, Y., Kim, D.-G., Ma, Y., Ren, Z., Niu, D., & Fu, H. (2025). Microbial responses to stoichiometric imbalances regulate soil organic carbon loss in degrading alpine ecosystems. Geoderma, 463. https://doi.org/10.1016/j.geoderma.2025.117560
42.Zhang, M., Li, Z., Zhang, B., Zhang, R., & Xing, F. (2024). Planting grass enhances relations between soil microbes and enzyme activities and restores soil functions in a degraded grassland. Front Microbiol, 15, 1290849. https://doi.org/10.3389/fmicb.2024.1290849
43.Zhang, M., Wen, J., Wang, X., Zhao, C., Ma, Y., Ma, Y., Li, S., & Cheng, X. (2025). Rest-grazing in spring affects the soil microbial community mainly by altering plant diversity. Journal of Plant Ecology, 18(1). https://doi.org/10.1093/jpe/rtae110
44.Zhang, W., Liu, Y., Geng, M., Chen, R., Wang, J., Xue, B., Xie, P., & Wang, J. (2022). Extracellular enzyme stoichiometry reveals carbon and nitrogen limitations closely linked to bacterial communities in China's largest saline lake. Front Microbiol, 13, 1002542. https://doi.org/10.3389/fmicb.2022.1002542
45.Angelovičová, L., & Fazekašová, D. (2014). Contamination of the soil and water environment by heavy metals in the former mining area of Rudňany (Slovakia). Soil and Water Research, 9(1), 18–24. https://doi.org/10.17221/24/2013-SWR
