Characterization of the microorganisms of human pus samples- a tertiary care hospital based study

  • Sadia Islam Sadia Islam, Junior Scientific Officer, Bangladesh Specialized Hospital Limited (BSHL)
  • Zarin Tahsin Aziz Zarin Tahsin Aziz, Research Assistant, Bangladesh University of Health Sciences (BUHS)
  • Md. Ashiqur Rahman Md. Ashiqur Rahman, Lab. Scientific Officer, Novus Clinical Research Services Limited (NCRSL)
  • Shohanur Rahaman Shohanur Rahaman, Scientific Officer, BIHS General Hospital
  • Md. Biplob Hossain Md. Biplob Hossain, Scientific Officer, BIHS General Hospital
Keywords: staphylococcus aureus; escherichia coli; pus sample; pyogenic

Abstract

The term "pyogenic infection" describes an infectious agent-induced localized inflammation and pus production caused by a buildup of dead leukocytes. 120 patients' specimens were gathered and grown on blood agar and MacCokey agar. Biochemical tests were used to identify colonies grown in these conditions, and subsequently their antibiotic susceptibility was examined. When combined with the patient's medical history, these data showed how common pyogenic infections were in BIHS General hospital, Dhaka. Within 86.67% of the Positive pyogenic infection were due to bacterial agents, with Staphylococcus aureus (23.07%), Escherichia coli (15.39%), Klebsiella (15.39%), Proteus (15.39%), Pseudomonas (7.69%), Enterococci (7.69%), Citrobacter (7.69%), Enterobacter (7.69%) being the major pathogens. Female was predominant than male which was 72 (60%) cases and 48 (40%) cases respectively. The most common age group for both the male and female groups was 40–60 years old, with 16 (13.33%) and 64 (53.33%) instances, respectively.  In conclusion, Staphylococcus aureus is the most often isolated bacterium following aerobic culture of pus. Nonetheless, there are fewer gram-positive cocci than gram-negative bacilli.

References

1. Khanam RA, Islam MR, Sharif A, Parveen R, Sharmin I, Yusuf MA. Bacteriological profiles of pus with antimicrobial sensitivity pattern at a teaching hospital in Dhaka City. Bangladesh J. 2018 Jun 11;5(1).

2. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. Diagnostic microbiology. The nonfermentative gram-negative bacilli. Philedelphia: Lippincott-Raven Publishers. 1997:253-320.

3. Cogen AL, Nizet V, Gallo RL. Skin microbiota: a source of disease or defence. British journal of dermatology. 2008 Mar 1;158(3):442-55.

4. Sartelli M, Malangoni MA, May AK, Viale P, Kao LS, Catena F, Ansaloni L, Moore EE, Moore FA, Peitzman AB, Coimbra R. World Society of Emergency Surgery (WSES) guidelines for management of skin and soft tissue infections. World journal of emergency surgery. 2014 Dec; 9:1-8.

5. Scalise A, Bianchi A, Tartaglione C, Bolletta E, Pierangeli M, Torresetti M, Marazzi M, Di Benedetto GI. Microenvironment and microbiology of skin wounds: the role of bacterial biofilms and related factors. InSeminars in vascular surgery 2015 Sep 1 (Vol. 28, No. 3-4, pp. 151-159). WB Saunders.

6. Hanif E, Hassan SA. Evaluation of antibiotic resistance pattern in clinical isolates of Staphylococcus aureus. Pak J Pharm Sci. 2019 Jul 1;32(4):1749-53.

7. Klein EY, Tseng KK, Pant S, Laxminarayan R. Tracking global trends in the effectiveness of antibiotic therapy using the Drug Resistance Index. BMJ global health. 2019 Apr 1;4(2): e001315.

8. Choy CY, Hsu LY. World antibiotic awareness week. Ann. Acad. Med. Singap. 2017 Nov 1; 46:413-4.

9. Brooks GF, Butel JS, Stephen A. Medical microbiology 23th edition.

10. Ananth S, Induja M, Thangamathi P, Prabha D, Vinotha K. In vitro antibacterial activity of biogenic gold nanoparticles from Murraya koenigii seed extract against pathogens associated with traumatic wound infections. Int. J. Fauna Biol. Stud. 2018; 5:137-44.

11. Omoyibo EE, Oladele AO, Ibrahim MH, Adekunle OT. Antibiotic susceptibility of wound swab isolates in a tertiary hospital in Southwest Nigeria. Annals of African medicine. 2018 Jul;17(3):110.

12. Shinagawa N, Iwasaki M. Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--Special references to bacteria isolated between July 1982 and March 2012. The Japanese Journal of Antibiotics. 2015 Jun 1;68(3):151-87.

13. Verma P. A study on isolation of different type of bacteria from pus. Int J Pharm Life Sci. 2012 Nov;3(11):2107-10.

14. Khanam RA, Islam MR, Sharif A, Parveen R, Sharmin I, Yusuf MA. Bacteriological profiles of pus with antimicrobial sensitivity pattern at a teaching hospital in Dhaka City. Bangladesh J. 2018 Jun 11;5(1).

15. Rajput K, Johri S, Goyal A. Identification of Bacterial Isolates and Their Antimicrobial Susceptibility Pattern from Wound/Pus Sample in a Tertiary Care Hospital, Gwalior, India. Annals of the National Academy of Medical Sciences (India). 2023 Jun 16.

16. Singh A, Verma V, Singh R. Antibiotic sensitivity pattern of pathogens isolated from pus culture-A tertiary care hospital based study. Journal of Nepalgunj Medical College. 2019 Dec 31;17(2):70-4.

17. Saha SK, Rahman MA, Mahmud MS, Islam MT, Islam MN, Islam S, Rahaman S, Zafreen A, Islam MR, Ali MS. Isolation and Characterization of Bacteriophage against Drug-resistant Staphylococcus aureus.
Published
2024-07-02
Section
Articles