Homologous postbiotic-synergized tri-strain composition for immune-response modulation and intestinal barrier restoration
Anas Ziraoui
European Life Science Research Association
Arjun Patel
European Life Science Research Association
Manon Petit
European Life Science Research Association
DOI: https://doi.org/10.59429/pest.v8i2.14489
Keywords: lactobacillus acidophilus; lactobacillus plantarum; bifidobacterium longum; postbiotics; immune modulation; intestinal barrier
Abstract
We investigated a tri-strain composition for immune-response modulation and intestinal barrier restoration built around Lactobacillus acidophilus LA-06, Lactobacillus plantarum LPL28, and Bifidobacterium longum BL21, each paired with a homologous postbiotic fraction. The platform used a fixed live-bacteria-to-postbiotic mass ratio of 1:2 and batch-matched fermentation supernatant as the sole protectant for the live fraction. In the dextran sulfate sodium colitis dataset, the three implementation examples reduced aggregate serum inflammatory mediators by 68.2%, 65.7%, and 67.4%, while increasing colonic tight-junction protein expression by 42.6%, 40.1%, and 41.8%; the live-bacteria-only comparator achieved 32.5% and 18.3%. Published evidence aligns with this direction, supporting barrier reinforcement, cytokine suppression, and the growing relevance of postbiotic preparations. The composition is therefore best understood as a barrier-centered platform in which strain complementarity, homologous postbiotic synergy, additive minimization, and reproducible anti-inflammatory performance define its scientific value.
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