Prevalence and Antibiotic Susceptibility Pattern of Enterococcus Spp. Isolated from Urine Samples
Md. SujonAli
Senior Executive, Joint Venture Medical Industry (JMI), Bangladesh
Md. AshiqurRahman
Lab. Scientific Officer, Novus Clinical Research Services Limited (NCRSL), Bangladesh
Sadia Islam
Junior Scientific Officer, Bangladesh Specialized Hospital Limited (BSHL), Bangladesh
Shohanur Rahaman
Scientific Officer, Diabetic Association of Bangladesh (BADAS), Bangladesh
KM. Sakib
Scientific Officer, ZH Sikder Womens Medical College and Hospital, Bangladesh
Md. Al-AminHossen
Clinical Biochemist, United Hospital Limited, Bangladesh
Adiatuj JahanRimu
MPH Student, University of South Asia, Bangladesh
DOI: https://doi.org/10.59429/mmr.v1i1.134
Keywords: Enterococcus spp, E.Coli, Linezolid, Penicillin, UTI.
Abstract
Enterococci are the most common cause of healthcare-associated urinary tract infections. Enterococcus spp. were isolated from each of Urine and differences for Enterococcus contamination in urine were determined. Because of its unimportant commensals, Enterococci have developed into diverse, deadly diseases due to the routinely prescribed antibiotics for the treatment of nosocomial UTIs are less effective, the developing antibiotic resistance is concerning. This study aimed to determine the prevalence and antibiotic susceptibility patterns of different enterococcal species isolated from urine samples and to observe the prevalence of microbes in urine samples among the different types of patients. The frequency of enterococci isolation from hospitalized patients' urinary tract has risen. This cross-sectional study was conducted from March 2021 to December 2021 at the Department of Microbiology, AMZ Hospital Dhaka, Bangladesh. A total of 439 urine samples were collected and 96 (21.87%) samples showed growth of different isolates, in growth isolates 29 (30.20%) samples were Enterococcus spp. In this study, the sample was sensitive against Linezolid (95.55%), Vancomycin (89.67%), Penicillin (68.98%), Gentamycin (68.96%), Imepenum (55.17%). From our study revealed that different strains of Enterococcus spp. are resistant to drugs at different rates.
References
1. Abedinn MZ, Huda MN Bacteremic Urinary Tract Infection and in vitro Antimicrobial Responsiveness Patterns at AMZ Hospital Ltd., Dhaka KYAU Journal, 5(2), 1-6.
2. Manero A, Blanch AR. Identification of Enterococcus spp. with a biochemical key. Applied and environmental microbiology. 1999 Oct 1;65(10):4425-30.
3. Devriese LA, Pot B, Collins MD. Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. Journal of Applied Bacteriology. 1993 Nov;75(5):399-408.
4. Ferede ZT, Tullu KD, Derese SG, Yeshanew AG. Prevalence and antimicrobial susceptibility pattern of Enterococcus species isolated from different clinical samples at Black Lion Specialized Teaching Hospital, Addis Ababa, Ethiopia. BMC research notes. 2018 Dec; 11:1-6.
5. Dargere S, Vergnaud M, Verdon R, Saloux E, Le Page O, Leclercq R, Bazin C. Enterococcus gallinarum endocarditis occurring on native heart valves. Journal of clinical microbiology. 2002 Jun;40(6):2308-10.
6. Effebi KR, Ballet GT, Seka MA, Baya DT, N’takpe BL. Physicochemical and microbiological characterization of human faeces and urine from composting toilets in Abidjan, Côte d’Ivoire. Environmental technology. 2019 Jan 28;40(3):293-301.
7. Tortora GJ, Funke BR, Case CL. Microbiology: an introduction. (No Title). 2004 Jan.
8. Felmingham D, Wilson AP, Quintana AI, Grüneberg RN. Enterococcus species in urinary tract infection. Clinical infectious diseases. 1992 Aug 1;15(2):295-301.
9. Ronald A. The etiology of urinary tract infection: traditional and emerging pathogens. The American journal of medicine. 2002 Jul 8;113(1):14-9.
10. Muhammad A, Khan SN, Ali N, Rehman MU, Ali I. Prevalence and antibiotic susceptibility pattern of uropathogens in outpatients at a tertiary care hospital. New Microbes and new infections. 2020 Jul 1; 36:100716.
11. PA W. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI document M100-S20. 2010.
12. Sammon JD, Sharma P, Rahbar H, Roghmann F, Ghani KR, Sukumar S, Karakiewicz PI, Peabody JO, Elder JS, Menon M, Sun M. Predictors of admission in patients presenting to the emergency department with urinary tract infection. World journal of urology. 2014 Jun; 32:813-9.
13. Pezeshki Najafabadi M, Dagoohian A, Rajaie S, Zarkesh-Esfahani SH, Edalati M. Common microbial causes of significant bacteriuria and their antibiotic resistance pattern in the Isfahan Province of Iran. Journal of Chemotherapy. 2018 Nov 17;30(6-8):348-53.
14. Tandogdu Z, Wagenlehner FM. Global epidemiology of urinary tract infections. Current opinion in infectious diseases. 2016 Feb 1;29(1):73-9.
15. Nicolle LE. A practical guide to antimicrobial management of complicated urinary tract infection. Drugs & aging. 2001 Apr; 18:243-54.
16. Boccella M, Santella B, Pagliano P, De Filippis A, Casolaro V, Galdiero M, Borrelli A, Capunzo M, Boccia G, Franci G. Prevalence and antimicrobial resistance of Enterococcus species: a retrospective cohort study in Italy. Antibiotics. 2021 Dec 19;10(12):1552.
17. Linden P, Miller C. Vancomycin-resistant enterococci: the clinical effect of a common nosocomial pathogen. Diagnostic microbiology and infectious disease. 1999 Feb 1;33(2):113-20.
18. Franz CM, Stiles ME, Schleifer KH, Holzapfel WH. Enterococci in foods—a conundrum for food safety. International journal of food microbiology. 2003 Dec 1;88(2-3):105-22.
19. Mahmud MS, Rahman MA, Islam MN, Islam S, Saha SK, Islam MT, Khatun A, Adhikari S. Reduction of Salmonella enterica & Staphylococcus aureus Biofilm Development on Glass Tube by Plant Extracts. Asian Journal of Research in Medical and Pharmaceutical Sciences. 2023 Nov 8;12(4):122-38.
20. Eaton TJ, Gasson MJ. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Applied and environmental microbiology. 2001 Apr 1;67(4):1628-35.
21. Barros M, Martinelli R, Rocha H. Enterococcal urinary tract infections in a university hospital: clinical studies. Brazilian Journal of Infectious Diseases. 2009; 13:294-6.
22. Ali S, Mirza IA, Yaqoob S, Hussain A, Khan I, Rafiq MY. Antimicrobial susceptibility pattern of enterococcus species isolated from patients with urinary tract infection. Gomal Journal of Medical Sciences. 2014 May 15;12(1).
23. Shinde RS, Koppikar GV, Oommen S. Characterization and antimicrobial susceptibility pattern of clinical isolates of Enterococci at a tertiary care hospital in Mumbai, India. Annals of Tropical Medicine & Public Health. 2012 Mar 1;5(2).
24. Saha SK, Rahman MA, Mahmud MS, Islam MT, Islam MN, Islam S, Nabilah S, Rahaman S, Zafreen A, Islam MR, Ali MS. Isolation and Characterization of Bacteriophage against Drug-resistant Staphylococcus aureus. Journal of Advances in Microbiology. 2023 Oct 12;23(10):128-38.