Viral Infections and Cancer Research

Isolation identification & antibiotic sensitivity of pus & tracheal aspirate isolates among tertiary hospital patients

Jannatul FerdousNazia

Jannatul Ferdous Nazia, Department of Microbiology and Immunology, Bangladesh University of Health Sciences (BUHS), xannatulnazia31@gmail, Orcid I’d: 0009-0007-9307-500X

Abu BakarSiddik

Abu Bakar Siddik, Department of Microbiology, Jagannath University, Bangladesh, absiddik5800@gmail.com, Orcid id: 0009-0007-9913-973X

Md. Al-AminHossen

Md. Al-Amin Hossen, Department of Biochemistry & Cell Biology, Bangladesh University of Health Sciences (BUHS), alaminhossainrocky@gmail.com, Orcid I’d: 0009-0007-7899-5967

Adiatuj JahanRimu

Adiatuj Jahan Rimu, Department of Public Health & Life Sciences, University of South Asia, Bangladesh, adiatujrimu@gmail.com, Orcid id :0009-0005-2647-177X

Tasnim Shamrin

Tasnim Shamrin, Department of Microbiology, Jagannath University, Bangladesh, tasnimshamrin@gmail.com, Orcid Id: 0009-0008-5323-8158

Sadia Islam

Sadia Islam, Department of Biochemistry & Cell Biology, Bangladesh University of Health Sciences (BUHS), sadia.buhs.6700@gmail.com, Orcid id: 0009-0007-7932-3804

Hazera AkterMukta

Hazera Akter Mukta, Department of Applied Laboratory Sciences, Bangladesh University of Health Sciences (BUHS), mohonamukta0288@gmail.com, Orcid id:0009-0008-0603-1701

Md. AshiqurRahman

Md. Ashiqur Rahman, Department of Biochemistry & Cell Biology, Bangladesh University of Health Sciences (BUHS), ararashiqur@gmail.com, Orcid id: 0000-0002-9430-5547

Keywords: Extensively drug resistant (XDR); Multidrug resistant (MDR); Antibiotic; Gram Positive Bacteria; Gram Negative bacteria

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Abstract

Background: The study aims to identify bacterial isolates and drug susceptibility patterns from patients with pus and wound discharge, addressing the issue of antibiotic resistance and the need for rational use in controlling infections. Methods and Materials: The cross sectional study at Bangladesh University of Health Sciences involved purposive sampling and Pus & tracheal aspirates from patients. It followed standard laboratory procedures for bacterial species identification and antimicrobial susceptibility testing using disk diffusion method following CLSI guidelines 2017. Results: This study examined 400 samples over a year, with most being pus (84%) and tracheal aspirate (16%). Males were predominant (56%), and gram-negative bacteria were predominant (74%). Staphylococcus aureus was the most sensitive to tigecycline (83%), followed by Meropenem & Doxycycline (67%), Gentamicin (58%), Cotrimoxaxole, Chloramphenicol & Colistin (42% each). Klebsiella pneumoniae were 100% sensitive to Meropenem, Ciprofloxacin & Tigecycline, and were 100% resistant to Cefotaxime, Cefixime & Cotrimoxazole. Escherichia coli were highly sensitive to Meropenem & Tigecycline, followed by Ceftazidime (69%), Gentamicin (63%), Ciprofloxacin & Colistin (62%), Doxycycline, Cefotoxime, Cefoxitin, Cotrimoxazole & Chloramphenicol (50%), Cefixime (31%), Amoxycillin (25%), and Ampicillin (24%). Staphylococcus aureus were most resistant to Vancomycin & Linezolid (75%). Multidrug resistance was found in 320 (80%) organisms in pus & tracheal aspirate. Conclusion: Multiple organisms were isolated from tracheal aspirate and pus, with most being multidrug resistant. The appropriate antibiotic for treatment should be chosen based on culture sensitivity.

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