Published
2025-03-10
Section
Articles
How to Cite
Prevalence and Antibiotic Resistance Patterns of Uropathogenic Bacteria in Urine Samples: A Study from Bangladesh
Kazi Riti
Kazi Mahjabin Hossain Riti, Department of Microbiology & Immunology, Bangladesh University of Health Sciences (BUHS), kazimahjabinhossain@gmail.com
Farzana IslamEva
Farzana Islam Eva, Department of Microbiology & Immunology, Bangladesh University of Health Sciences (BUHS), farzanaislameva1@gmail.com
Nisat Sultana
Nisat Sultana, Department of Microbiology, Stemz Health Care Limited, Bangladesh, nisat1995liza@gmail.com
Md. SujonAli
Md. Sujon Ali, Department of Medical Biotechnology, University of Technology Sydney (UTS), Australia, msujonali07@gmail.com
Md. AshiqurRahman
Md. Ashiqur Rahman, Department of Laboratory Medicine, Novus Clinical Research Services Limited, Dhaka, Bangladesh, ararashiqur@gmail.com
Sadia Islam
Sadia Islam, Department of Laboratory Medicine, Bangladesh Specialized Hospital PLC, Bangladesh, sadia.buhs.6700@gmail.com
Keywords: Urinary Tract Infection; Escherichia coli; Klebsiella pneumoniae; Antimicrobial Resistance; ESBL; AmpC beta-lactamase
Abstract
Introduction: Urinary tract infections (UTIs) are one of the most common bacterial infections globally, with Escherichia coli being the predominant causative agent. The rise in antimicrobial resistance (AMR) among uropathogens, particularly to commonly prescribed antibiotics, has become a critical public health concern. In Bangladesh, inappropriate use of antibiotics has contributed to the emergence of multidrug-resistant (MDR) pathogens, making it essential to monitor local resistance patterns. This study aims to isolate and identify the bacterial pathogens responsible for UTIs and assess their antimicrobial susceptibility profiles. Methodology: This cross-sectional study was conducted at a local hospital in Bangladesh from November 2023 to May 2024. A total of 50 urine samples were collected from patients with clinical symptoms of UTIs. Isolates were identified using standard microbiological methods, including Gram staining and biochemical tests. The antimicrobial susceptibility of the isolates was evaluated using the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. The antibiotics tested included imipenem, doxycycline, ciprofloxacin, cefoxitin, ampicillin, and others. Extended-Spectrum Beta-Lactamase (ESBL) and AmpC beta-lactamase production were also determined. Results: Out of 50 urine samples, 40 (80%) were culture-positive. Escherichia coli was the most prevalent pathogen, accounting for 61.1%, followed by Klebsiella pneumoniae (33.3%). Both pathogens showed 100% sensitivity to imipenem, but exhibited varying levels of resistance to other antibiotics, with E. coli showing 16.7% resistance to ampicillin. ESBL production was observed in 44.4% of isolates, with K. pneumoniae showing higher rates (66.7%). AmpC beta-lactamase production was noted in 33.3% of E. coli isolates. Conclusion: The study highlights the dominance of E. coli and K. pneumoniae in UTIs, with significant AMR, especially to beta-lactam antibiotics. Continuous surveillance and appropriate antimicrobial stewardship are critical to managing rising resistance and improving treatment outcomes.
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